Tuesday, 20 September 2011

Biotinylation of Targets

Hey guys, so I was about to start the selection for my target this semester but realized that it's not a guarantee whether my target (KRAS) is biotinylated or not. This is obviously important because I want to know whether I should perform a bead or filter selection for this target. But this question probably applies to everyone's targets, to what extent should we trust the labeling on the freezer for biotinylated and non-biotinylated?

Thanks,

Ryan

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